Targeting membrane proteins to inner segments of vertebrate photoreceptors
نویسندگان
چکیده
..................................................................................................................... 25 Introduction ................................................................................................................ 26 Materials and Methods ............................................................................................... 28 Animals ................................................................................................................... 28 Immunohistochemistry ............................................................................................ 28
منابع مشابه
Intracellular pH modulates inner segment calcium homeostasis in vertebrate photoreceptors.
Neuronal metabolic and electrical activity is associated with shifts in intracellular pH (pH(i)) proton activity and state-dependent changes in activation of signaling pathways in the plasma membrane, cytosol, and intracellular compartments. We investigated interactions between two intracellular messenger ions, protons and calcium (Ca²(+)), in salamander photoreceptor inner segments loaded with...
متن کاملThe Extractability of Inner-Membrane Proteins from Salmonella typhimurium Intact Cells, Spheroplasts and Inner-Membrane Fragments by Non-Denaturing Detergents
The effect of Triton X-100, Na cholate and Tween 80 on the solubilization of integral membrane proteins in intact cells, spheroplasts and inner-membrane fragments of Salmonella typhimurium was studied. The detergents were used in various concentrations (1.6 to 64 mM) and cytochromes b and d were used as marker to monitor the solubilization of membrane-bound proteins. Results showed that no inne...
متن کاملCompartmentalization of Calcium Extrusion Mechanisms in the Outer and Inner Segments of Photoreceptors
Differential localization of calcium channel subtypes in divergent regions of individual neurons strongly suggests that calcium signaling and regulation could be compartmentalized. Region-specific expression of calcium extrusion transporters would serve also to partition calcium regulation within single cells. Little is known about selective localization of the calcium extrusion transporters, n...
متن کاملOpposing effects of calcium and barium in vertebrate rod photoreceptors.
Intracellular recording in outer segments of red rods of the toad retina showed that increasing Ca(2+) concentration in the perfusate mimicked certain aspects of light adaptation. Light sensitivity was reduced, the amplitude of light responses was reduced, the time course of light responses was altered by shortening the delay to the peak and increasing the decay rate, and the resting membrane p...
متن کاملLight-Mediated Activation of Rac-1 in Photoreceptor Outer Segments
Small GTP binding proteins regulate diverse biological processes including gene expression, cytoskeleton reorganization, and protein and vesicular transport. While small GTPases have been investigated in a wide variety of cells, few studies have addressed their role in photoreceptors. In vertebrate retinal rods, the light stimulus is transmitted from rhodopsin via the pathway mediated by the he...
متن کامل